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superscript™ iii rt cdna synthesis kit  (Thermo Fisher)


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    Thermo Fisher superscript™ iii rt cdna synthesis kit
    Superscript™ Iii Rt Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/superscript™ iii rt cdna synthesis kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    superscript™ iii rt cdna synthesis kit - by Bioz Stars, 2026-06
    90/100 stars

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    Overexpression of Mash-1 promotes neuronal differentiation of BMSCs. (a) RT-PCR (left) and western blot (right) representation of selected markers from cDNA microarray which showed consistencies with the data as analyzed. (b) Distribution of neuronal differentiation markers. Immunofluorescence staining using specific antibodies against GAP43, Mash-1, and Hes5 was performed in BMSCs with bFGF treatment for 24 hours. NC: negative control. Images are representatives of at least three experiments. (c) Mash-1 overexpressing BMSCs showed a typical neuronal morphology. (d) Western blotting analysis showed a significant increase of the NSE expression in Mash-1 overexpressing BMSCs compared to control cells. (e) The expression of SV2a and SNAP-25 were determined by qRT-PCR in Mash-1 overexpressing BMSCs. Data of RT-PCR indicate the mean ± SEM of three experiments.

    Journal: Stem Cells International

    Article Title: Gene Profiles in the Early Stage of Neuronal Differentiation of Mouse Bone Marrow Stromal Cells Induced by Basic Fibroblast Growth Factor

    doi: 10.1155/2020/8857057

    Figure Lengend Snippet: Overexpression of Mash-1 promotes neuronal differentiation of BMSCs. (a) RT-PCR (left) and western blot (right) representation of selected markers from cDNA microarray which showed consistencies with the data as analyzed. (b) Distribution of neuronal differentiation markers. Immunofluorescence staining using specific antibodies against GAP43, Mash-1, and Hes5 was performed in BMSCs with bFGF treatment for 24 hours. NC: negative control. Images are representatives of at least three experiments. (c) Mash-1 overexpressing BMSCs showed a typical neuronal morphology. (d) Western blotting analysis showed a significant increase of the NSE expression in Mash-1 overexpressing BMSCs compared to control cells. (e) The expression of SV2a and SNAP-25 were determined by qRT-PCR in Mash-1 overexpressing BMSCs. Data of RT-PCR indicate the mean ± SEM of three experiments.

    Article Snippet: Total cellular RNA was isolated using Trizol reagent (Invitrogen) and reverse transcribed using Invitrogen's First Strand cDNA Synthesis Superscript III RT Kit.

    Techniques: Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Microarray, Immunofluorescence, Staining, Negative Control, Expressing, Quantitative RT-PCR